The aim of this study was to scope and pilot ecosystem accounts for a selected suite of protected and other land areas that comprise a variety of habitats and ecosystem services. The project tested and applied spatially disaggregated accounting methodologies which are crucial to the successful development of natural capital accounting, one of Defra’s commitments under the Natural Environment White Paper. The ecosystem accounts are intended to provide a valuable resource to help inform local and regional ecosystem resource management decisions within the pilot areas. We were involved in selecting and liaising with the six pilot areas (3 National Parks, 1 Area of Outstanding Natural Beauty and 2 Land Use Strategy areas). We also led on the collation and analysis of data linked to crops and livestock production and the resource rent calculations for the agriculture.